HTTP Error 401.3 - Unauthorized: Access is denied
This error may occur if the file you are trying to access does not have the appropriate user permissions applied. To ensure each file in your site's wwwroot folder has the appropriate permissions, use the following steps.
Please note that this will replace each file and folder inside the wwwroot folder with the permisssions that the wwwroot folder currently has applied to it.
Navigate to the site's wwwroot folder, right-click on the folder and select Properties.

Click the Advanced button.

Check the
Replace permissions.. checkbox and click
Apply.

You will be prompted with a warning, click Yes.